Chromatin immunoprecipitation followed by next-generation sequencing (ChIP-Seq) is one of the most commonly used tools in epigenetics research. This webinar will discuss approaches to the technique of ChIP-Seq to help ensure a successful experiment.

As scientists continue to learn more about the biology of aging, the role of epigenetics in regulating the aging process has become evident. In this webinar, Dr. Sarantis Chlamydas, Ph.D., Epigenetics Expert at Active Motif, discusses the epigenetic mechanisms involved in aging and highlights the available tools and strategies for studying these mechanisms.

Epigenetic techniques have typically been difficult to use in a clinical setting. In this free webinar, Dr. Joshua Messinger, Ph.D., R&D Scientist at Active Motif discusses innovative approaches that have been developed to enable epigenetic analysis to be applied using clinical samples.

Epigenetic tools and techniques provide a valuable avenue to understanding the underlying mechanisms of disease. In this webinar, Dr. Michael Garbati, Ph.D., Technical Support Scientist at Active Motif, discusses how epigenetic methods can be applied to study specific disease states, using the specific example of blood cancers including chronic lymphocytic leukemia (CLL).

Achieving proper sonication of chromatin and DNA, from a wide array of sample types, is critical for the success of several of today’s most popular lab techniques. Limitations in traditional shearing approaches can lead to experimental failures and inconsistent results. Therefore, Active Motif has developed a new instrument to address those issues.

In this webinar, Dr. Rwik Sen, Ph.D., Field Applications Scientist at Active Motif, discusses how the PIXUL™ Multi-Sample Sonicator addresses many of the issues around chromatin and DNA shearing.

The Epigenetics of the Exposome and Aging

Aging results in a gradual and progressive loss of physiological functions and physical abilities over time. Overcoming the growing burden of age-related issues is one of the greatest challenges facing modern society. These issues are leading to a major drain on healthcare resources worldwide, so a better understanding of the biology of aging is critically important.

In this webinar, Dr. Paul Shiels, Ph.D., Professor of Epigenetics at the University of Glasgow, discusses how our behavior and the environment impact health and aging at the molecular level.

Traditionally, Histone PTM levels are measured by western blots or ELISAs, but these methods require large amounts of material and can only examine one histone modification at a time.

In this webinar, Mary Anne Jelinek Ph.D., Senior Research Scientist at Active Motif will discuss our Histone H3 PTM Multiplex Assay and its advantages over traditional western blotting.

Although ChIP-Seq has contributed greatly to our understanding of the epigenome and gene regulation, it is not without its limitations, which can impede our ability to answer complex scientific questions.

In this webinar, Adam Blattler Ph.D., Research Scientist at Active Motif, will discuss the variety of tools and services we have developed to overcome many of these challenges.

Mutations that lead to changes in gene expression are common in many human diseases. Therefore, a thorough understanding of the mechanisms regulating or mis-regulating gene expression is crucial to understanding disease initiation and progression. ChIP-Seq is the predominant method used to analyze DNA-protein interactions, but it can have limited resolution in identifying a transcription factor binding site.

In this webinar, Bryce Alves, Ph.D., Research Scientist at Active Motif will discuss ChIP-Exo, a powerful technique for high-resolution mapping of transcription factor binding sites.

An ongoing problem in ChIP-Seq data analysis is the often large discrepancy between expected changes in histone marks and the actual ChIP-Seq data when using drug-treated cells.

In this webinar, Brian Egan, Ph.D., Epigenetic Services Manager at Active Motif, discusses a novel ChIP-Seq spike-in normalization strategy and shows how normalization of ChIP-Seq data using this approach reveals the expected changes in the final ChIP-Seq data set for inhibitor-treated samples.

Chromatin Immunoprecipitation (ChIP) is a powerful tool for studying protein-DNA interactions, but it can be technically challenging. To date, ChIP studies have been mainly limited to cultured cells and model systems.