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PLXDC2 is a new key marker for mouse & human hematopoietic stem cells (HSCs) isolation

Hematopoietic stem cells (HSCs) give rise to other blood cells. HSCs are defined by the capabilities of multi-lineage differentiation and long-term self-renewal, allowing the restoration of hematopoiesis after insults, such as infections or therapeutic ablation. Different markers (multi-antibody combination) are used for positive and negative selection (CD34⁺CD38^-CD90⁺CD45RA^-CD49f⁺) as a conventional protocol to isolate a population of cells that results in only ~10% functional HSCs when human cord blood is used as the starting material. So new markers are required to purify functional human or mouse HSCs in an easier and more efficient way.

Recently, the lab of Prof. Toshio Kitamura (University of Tokyo, Japan) described in the journal Communications Biology that PLXDC2 is highly expressed in human and mouse HSCs derived from both cord blood and bone marrow, and PLXDC2 can be used as a new key marker to enrich both human and mouse HSCs using flow cytometry. PLXDC2 is a single-pass type I membrane protein that plays important functions during nervous system development and in the modulation of the immune system, but the function of PLXDC2 in the hematopoietic system is unknown. They showed that the use of both PLXDC2 and CD150 membrane proteins is sufficient for the isolation of functional HSCs without any additional markers. PLXDC2 in combination with CD150 is a new, very efficient, and easy protocol to highly purify mouse and human functional HSCs compared with the conventional multi-antibody combination.

AdipoGen Life Sciences provides unique human and mouse PLXDC2 monoclonal antibodies for flow cytometry, as well as proteins that can be used to study this new role of PLXDC2:

- PLXDC2 (mouse), mAb (Bonny-1) (Prod. No. AG-20B-0086)
- PLXDC2 (mouse), mAb (Bonny-1) (Biotin) (Prod. No. AG-20B-0086B)
- PLXDC2 (mouse), mAb (Bonny-1) (ATTO 647N) (Prod. No. AG-20B-0086TS)
- PLXDC2, mAb (Bonny-2) (Prod. No. AG-20B-0089)
- PLXDC2 (mouse):Fc (silent) InVivoKine™ (Prod. No. AG-40B-0270)
- PLXDC2 (mouse) (His) (rec.) (Prod. No. AG-40B-0271)
- PLXDC2 (human) (His) (rec.) (Prod. No. AG-40B-0275)
- PLXDC2 (human) ELISA Kit (Prod. No. AG-45B-0035)
- PLXDC2 (mouse) ELISA Kit (Prod. No. AG-45B-0036)

LIT: Prospective isolation of mouse and human hematopoietic stem cells using PLXDC2: Y. Tanaka, et al.; Commun. Biol. (Epub ahead of print) (2025)