TSLP Responsive Luciferase Reporter Ba/F3 Cell Line
BPS-82500 2 vials
Synonyms: Thymic stromal lymphopoietin, TSLP
Data Sheet: 
Applications
Screen and characterize modulators of TSLP activity.
Source / Host
mouse IL-3-dependent pro-B cell line, suspension.
Shipment
-80°C
Literature References
Ebina-Shibuya R. and Leonard WJ. 2023 Nat Rev Immunol, 23: 24 - 37
Marković I. and Savvides S., 2020 Front. Immunol., 11.
Palacios R. et al., 1984 Nature 309 (5964): 126-131.
Rochman Y., Spolski R. and Leonard, W., 2009 Nat Rev Immunol, 9:
Marković I. and Savvides S., 2020 Front. Immunol., 11.
Palacios R. et al., 1984 Nature 309 (5964): 126-131.
Rochman Y., Spolski R. and Leonard, W., 2009 Nat Rev Immunol, 9:
Description
The TSLP Responsive Luciferase Reporter Ba/F3 Cell Line is a murine Ba/F3 cell line engineered to express both TSLP-R (thymic stromal lymphopoietin receptor, also known as CRLF2, cytokine receptor like factor 2) (NM_022148.4) and IL-7Ra (Interleukin 7 receptor alpha) (NM_002185.5) separated by a self-cleaving P2A peptide. The construct was delivered bytransduction of STAT5 Luciferase Reporter Ba/F3 cells (#79772), which express a firefly luciferase reporter driven by STAT5 response elements located upstream of the minimal TATA promoter. After activation by TSLP, the endogenous transcription factor STAT5 binds to the response elements, inducing transcription of the luciferase reporter gene.
This cell line has been validated to respond to TSLP. Additional functional validation demonstrates that TSLP-induced luciferase activity can be inhibited by either anti-TSLP or anti-TSLPR neutralizing antibodies.
TSLP binds to TSLPR, recruits the IL-7Ra co-receptor and activates downstream JAK1/2 (janus kinase 1/2) tyrosine kinases, which phosphorylate the transcription factor STAT5. STAT5 phosphorylation triggers the formation of a homodimer and translocation to the nucleus, where it can activate the transcription of the Firefly luciferase reporter driven by STAT5 response elements present in the promoter. - Screen and characterize modulators of TSLP activity. - Avoid freeze/thaw cycles
ESCLUSIVAMENTE PER USO DI RICERCA (RUO) e non per uso terapeutico o diagnostico su uomini o animali. Il prodotto NON è un Dispositivo Medico o un Diagnostico in Vitro.
PRODUCT FOR RESEARCH USE ONLY (RUO) and not for therapeutic or diagnostic use on humans or animals. The product is NOT a Medical Device or an In-Vitro Diagnostic (IVD).
