SIRT5 (Sirtuin5) Fluorogenic Assay Kit
BPS-50085 96 reactions
Synonyms: sirt5, sirtuin 5
Data Sheet: 
Applications
Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
Shipment
-80°C (dry ice)
Literature References
1.J. Du et al. (2011) Science. 334: 806.
2. A. Madsen et al. (2012) J. Med. Chem 55: 5582.
3. F. Fisher et al. (2012) Plos One. 7: 1.
2. A. Madsen et al. (2012) J. Med. Chem 55: 5582.
3. F. Fisher et al. (2012) Plos One. 7: 1.
Description
The Fluorogenic SIRT5 Assay Kit is a complete assay system designed to measure
SIRT5 activity for screening and profiling applications. The Fluorogenic
SIRT5 Assay Kit is based on a unique fluorogenic substrate and developer combination.
This assay method eliminates dealing with the radioactivity, extraction, and
chromatography aspects of traditional assays. Using this kit, only three simple steps on
a microtiter plate are needed to analyze the SIRT5 activity level. First, the Sirtuin 5
fluorometric substrate (SIRT substrate 5), containing a succinylated lysine side chain, is
incubated with purified SIRT5 enzyme. The desuccinylation sensitizes the substrate so
subsequent treatment with the Lysine Developer produces a fluorophore that can then
be measured using a fluorescence reader. - Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications. - Avoid freeze/thaw cycles.
ESCLUSIVAMENTE PER USO DI RICERCA (RUO) e non per uso terapeutico o diagnostico su uomini o animali. Il prodotto NON è un Dispositivo Medico o un Diagnostico in Vitro.
PRODUCT FOR RESEARCH USE ONLY (RUO) and not for therapeutic or diagnostic use on humans or animals. The product is NOT a Medical Device or an In-Vitro Diagnostic (IVD).
