SIRT3 (Sirtuin3) Fluorogenic Assay Kit
BPS-50088 32 reactions
Synonyms: sirt3, sirtuin 3
Data Sheet: 
Applications
Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
Shipment
-80°C (dry ice)
Literature References
1. A. Ito et al. (2001) EMBO J. 20 1331.
2. A. Ito et al. (2002) EMBO J. 21 6236.
3. N.A. Barlev et al. (2001) Mol. Cell 8 1243. Application Reference(s):
Inhibition o
2. A. Ito et al. (2002) EMBO J. 21 6236.
3. N.A. Barlev et al. (2001) Mol. Cell 8 1243. Application Reference(s):
Inhibition o
Description
The Fluorogenic SIRT3 Assay Kit is a complete assay system
designed to measure Sirtuin 3 (SIRT3) activity for screening and profiling
applications. The Fluorogenic SIRT3 Assay Kit is based on
a unique fluorogenic substrate and developer combination. This assay method
eliminates dealing with the radioactivity, extraction, and chromatography aspects
of traditional assays. Using this kit, only two simple steps on a microtiter plate are
needed to analyze the SIRT3 activity level. First, the HDAC fluorometric
substrate (HDAC substrate 1), containing an acetylated lysine side chain, is
incubated with purified SIRT3 enzyme. The deacetylation sensitizes the substrate so
subsequent treatment with the Lysine Developer produces a fluorophore that can
then be measured using a fluorescence reader. - Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications. - Avoid freeze/thaw cycles.
ESCLUSIVAMENTE PER USO DI RICERCA (RUO) e non per uso terapeutico o diagnostico su uomini o animali. Il prodotto NON è un Dispositivo Medico o un Diagnostico in Vitro.
PRODUCT FOR RESEARCH USE ONLY (RUO) and not for therapeutic or diagnostic use on humans or animals. The product is NOT a Medical Device or an In-Vitro Diagnostic (IVD).
