Histone H4K20me3 antibody (mAb)
V13-39671 100 µg
Data Sheet: 
Reactivity
Human, Mouse, Wide Range Predicted
Applications
ChIP, DB, ICC, WB
Source / Host
Mouse
Immunogen
This Histone H4 trimethyl Lys20 antibody (mAb) was raised against a peptide containing trimethyl Lys20 of Histone H4.
Clone
6F8-D9
Shipment
Room Temp
Short Term Storage
-20C
Long Term Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years.
Description
Applications Validated by Active Motif: ChIP: 5 - 10 ug per ChIP WB*: 1 - 2 ug/ml dilution DB: 0.2 ug/ml dilution Slight reactivity towards histone H4 dimethyl Lys20 might be observed under certain conditions. Individual optimization may be required. *Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western Blot. The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer can be used to aid in detection by Western blot. Individual optimization may be required.
ESCLUSIVAMENTE PER USO DI RICERCA (RUO) e non per uso terapeutico o diagnostico su uomini o animali. Il prodotto NON è un Dispositivo Medico o un Diagnostico in Vitro.
PRODUCT FOR RESEARCH USE ONLY (RUO) and not for therapeutic or diagnostic use on humans or animals. The product is NOT a Medical Device or an In-Vitro Diagnostic (IVD).
