Histone H3K4me3 antibody (pAb)
V13-39159 100 µl
Data Sheet: 
Reactivity
Budding Yeast, Human, Mouse, Wide Range Predicted
Applications
ChIP, ChIP-Seq, CUT&RUN, CUT&Tag, DB, ICC, IF, IHC, WB
Source / Host
Rabbit
Immunogen
This Histone H3 trimethyl Lys4 antibody was raised against a peptide including trimethyl-lysine 4 of histone H3.
Shipment
Room Temp
Short Term Storage
-20C
Long Term Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years.
Description
Applications Validated by Active Motif: ChIP: 3 - 5 ul per ChIP ChIP-Seq: 3 ul each ICC/IF: 1:500 - 1:1,000 dilution WB: 1:500 - 1:2,000 dilution CUT&Tag: 1 ul per 50 ul reaction* CUT&RUN: 1 ul per 50 ul reaction *This antibody has been validated for CUT&Tag using Active Motif's CUT&Tag-IT Assay Kit, Catalog No. 53160. modENCODE validation: this antibody was validated for ChIP-Seq in this study (see reference). NGS-QC certification: this antibody has been processed by the NGS-QC generator. For additional details, click here.. **Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot.
ESCLUSIVAMENTE PER USO DI RICERCA (RUO) e non per uso terapeutico o diagnostico su uomini o animali. Il prodotto NON è un Dispositivo Medico o un Diagnostico in Vitro.
PRODUCT FOR RESEARCH USE ONLY (RUO) and not for therapeutic or diagnostic use on humans or animals. The product is NOT a Medical Device or an In-Vitro Diagnostic (IVD).
