Histone H3K27me3 antibody (pAb)
V13-39055 50 µg
Data Sheet: 
Reactivity
Human, Monkey, Mouse, Wide Range Predicted
Applications
ChIP, ChIP-Seq, CUT&RUN, CUT&Tag, DB, ICC, IF, IHC, WB
Source / Host
Rabbit
Immunogen
This antibody was raised against a peptide including trimethyl-lysine 27 of histone H3.
Shipment
Room Temp
Short Term Storage
-20C
Long Term Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years.
Description
Applications Validated by Active Motif: ChIP: 5 - 10 ug per ChIP ChIP-Seq: 5 ug each ICC/IF: 2 ug/ml dilution IHC(FFPE): 2 ug/ml dilution WB*: 0.5 - 2 ug/ml dilution CUT&Tag: 1 ug per 50 ul reaction* CUT&RUN: 1 ug per 50 ul reaction* *This antibody has been validated for CUT&Tag using Active Motif's CUT&Tag-IT Assay Kit, Catalog No. 53160. *Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for WB. For H3K27me3, we also offer AbFlex H3K27me3 Recombinant Antibody (rAb). For details, see Catalog No. 91167.
ESCLUSIVAMENTE PER USO DI RICERCA (RUO) e non per uso terapeutico o diagnostico su uomini o animali. Il prodotto NON è un Dispositivo Medico o un Diagnostico in Vitro.
PRODUCT FOR RESEARCH USE ONLY (RUO) and not for therapeutic or diagnostic use on humans or animals. The product is NOT a Medical Device or an In-Vitro Diagnostic (IVD).
