HSP90β (C-Terminal Domain) TR-FRET Kit
BPS-50262-1 96 reactions
Data Sheet: 
Applications
Screening inhibitors of HSP90β binding for drug discovery and HTS applications.
Determine compound IC50.
Perform real-time kinetic analyses.
Shipment
-80°C (dry ice)
Literature References
Mishra S., et al. The development of HSP90β-selective inhibitors to overcome detriments associated with pan-HPS90 inhibition. J Med Chem 64 (3), 1545-1557 (2021)
Khandelwal A., et al. Structure-guided design of an HPS90β N-terminal isoform-selective inhib
Description
The HSP90β (C-terminal domain) TR-FRET Assay Kit is a sensitive high-throughput screening (HTS) TR-FRET Assay Kit designed to measure the inhibition of HSP90β binding to its target protein PPDI in a homogeneous 96-well format or 384-well format. It utilizes a Terbium-labeled donor and a Dye-labeled acceptor to complete the FRET pairing. This TR-FRET based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications.
The Terbium-labeled donor will bind to PPDI, while the Dye-labeled acceptor will bind to HSP90β. When PPID is bound to HSP90β TR-FRET occurs and it can be measured using a fluorescence plate reader. - Screening inhibitors of HSP90β binding for drug discovery and HTS applications.
Determine compound IC50.
Perform real-time kinetic analyses. - Avoid freeze/thaw cycles
ESCLUSIVAMENTE PER USO DI RICERCA (RUO) e non per uso terapeutico o diagnostico su uomini o animali. Il prodotto NON è un Dispositivo Medico o un Diagnostico in Vitro.
PRODUCT FOR RESEARCH USE ONLY (RUO) and not for therapeutic or diagnostic use on humans or animals. The product is NOT a Medical Device or an In-Vitro Diagnostic (IVD).
