HDAC developer (2X)
BPS-50030 6 ml
Data Sheet: 
Applications
Study of HDAC kinetics, regulation and inhibitor sensitivity. Ideal for drug discovery and HTS applications and cell-based HDAC assays.
Shipment
-80°C (dry ice)
Literature References
1. K. Ito et al. Proc. Natl. Acad. Sci. USA 2002 99 8921.
2. B. Heltweg and M. Jung Anal. Biochem. 2002 302 175.
3. S. Milutinovic et al. J. Biol. Chem. 2002 277 20974 Application Reference:
2. B. Heltweg and M. Jung Anal. Biochem. 2002 302 175.
3. S. Milutinovic et al. J. Biol. Chem. 2002 277 20974 Application Reference:
Description
HDAC assay developer is an essential reagent for signal generation in HDAC assays performed with any of BPS Bioscience's HDAC fluorimetric substrates or assay kits. An appropriate fluorimetric substrate, comprising an acetylated lysine side chain, is first incubated with a sample containing HDAC activity (nuclear or cellular extract, purified enzyme, bead-bound immunocomplex, etc.). Deacetylation of the substrate sensitizes it so that, in a second step, treatment with the HDAC assay developer produces a fluorophore. Trichostatin A is included as an 'inhibitor stop' for class I and II HDACs. - Study of HDAC kinetics, regulation and inhibitor sensitivity. Ideal for drug discovery and HTS applications and cell-based HDAC assays. - Avoid freeze/thaw cycles. Use product undiluted.
ESCLUSIVAMENTE PER USO DI RICERCA (RUO) e non per uso terapeutico o diagnostico su uomini o animali. Il prodotto NON è un Dispositivo Medico o un Diagnostico in Vitro.
PRODUCT FOR RESEARCH USE ONLY (RUO) and not for therapeutic or diagnostic use on humans or animals. The product is NOT a Medical Device or an In-Vitro Diagnostic (IVD).
