HDAC8 Fluorogenic Assay Kit
BPS-50068 96 reactions
Synonyms: histone deacetylase, HDAC
Data Sheet: 
Applications
Great for studying enzyme kinetics and screening small molecular inhibitors of HDAC8 for drug discovery and HTS applications.
Shipment
-80°C (dry ice)
Literature References
1. Vijayakumar, B., et al. Bioinformation 2011; 7(3): 134-41.
2. Oehme, I., et al. Expert Opin Investig Drugs . 2009 Nov; 18(11): 1605-17. Application Reference(s):
1.
2. Oehme, I., et al. Expert Opin Investig Drugs . 2009 Nov; 18(11): 1605-17. Application Reference(s):
1.
Description
The Fluorogenic HDAC 8 Assay Kit is a complete assay system
designed to measure histone deacetylase (HDAC) 8 activity for screening and profiling
applications. It comes in a convenient 96-well format, with all the reagents necessary for
100 fluorescent HDAC activity measurements. In addition, the kit includes purified
HDAC8 and a potent HDAC inhibitor, Trichostatin A, for use as a positive and negative
control. The Fluorogenic HDAC 8 Assay Kit is based on a unique fluorogenic substrate
and developer combination. This assay method eliminates dealing with the radioactivity,
extraction, and chromatography aspects of traditional assays. Using this kit, only twoa
simple steps on a microtiter plate are needed to analyze the HDAC 8 activity level. First,
the HDAC fluorometric substrate is incubated with purified HDAC8 enzyme. The
deacetylation sensitizes the substrate so subsequent treatment with the Lysine
Developer produces a fluorophore that can then be measured using a fluorescence
reader. - Great for studying enzyme kinetics and screening small molecular inhibitors of HDAC8 for drug discovery and HTS applications. - Avoid freeze/thaw cycles.
ESCLUSIVAMENTE PER USO DI RICERCA (RUO) e non per uso terapeutico o diagnostico su uomini o animali. Il prodotto NON è un Dispositivo Medico o un Diagnostico in Vitro.
PRODUCT FOR RESEARCH USE ONLY (RUO) and not for therapeutic or diagnostic use on humans or animals. The product is NOT a Medical Device or an In-Vitro Diagnostic (IVD).
