Gram’s fuchsin Solution
CDX-G0064-L250 250 ml
Synonyms: Fuchsin solution; Basic Red 9; Magenta O; Basic Violet 14
Data Sheet: 
Source / Host
Synthetic
CAS
58969-01-0 (comp.)
MW
337.85
Shipment
Ambient
Short Term Storage
+20°C
Long Term Storage
+4°C
Shelf Life
Stable for at least 1 year after receipt when stored at +4°C.
Description
Chemical. CAS: 632-99-5. Formula: C20H20ClN3. MW: 337.85. Gram's Fuchsin Solution is a reagent that could be used in qualitative procedures to differentiate gram-negative from gram-positive organisms. The Gram staining allows a fast differentiation of bacteria in Gram-positive and Gram-negative. The mureine structure of the bacteria walls is the basis of the color affinity. Bacteria will be stained with Gram's crystal violet solution - an aniline dye - in the first step. After the treatment with iod solution, a dye-iod complex will form. During the decolorizing step, this complex stays in the multilayer mureine structures of the Gram-positive bacteria and they will appear blue/violet. Gram-negative bacteria have a monolayer mureine structure only, the dye-iod complex does not stay bound to the cellwall, they will be decolorized. Gram-negative bacteria will be counterstained by Gram's Fuchsin solution and appear red/pink. Both Gram-positive bacteria and Gram-negative bacteria pick up the counterstain. The counterstain, however, is unseen on Gram-positive bacteria because of the darker crystal violet stain.
ESCLUSIVAMENTE PER USO DI RICERCA (RUO) e non per uso terapeutico o diagnostico su uomini o animali. Il prodotto NON è un Dispositivo Medico o un Diagnostico in Vitro.
PRODUCT FOR RESEARCH USE ONLY (RUO) and not for therapeutic or diagnostic use on humans or animals. The product is NOT a Medical Device or an In-Vitro Diagnostic (IVD).
