APRIL (human) (H98) (multimeric) (rec.)

  AG-40B-0088-C100 100 ug

Data Sheet:     SDS:    

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Human, Mouse  

CHO cells  

Binds to human and mouse BCMA and TACI. Does not bind to proteoglycans.  

>95% (SDS-PAGE)  

~32kDa (SDS-PAGE)  

BI  

+4°C  

-20°C  

Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.  

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Description

 

Protein. The extracellular domain of human APRIL (aa 98-233) is fused at the N-terminus to human ACRP30headless (aa 16-108) and a FLAG®-tag. Source: CHO cells. Endotoxin content: <0.01EU/µg purified protein (LAL test; Lonza). Lyophilized. Contains PBS + 0.5% Trehalose. Binds to human and mouse BCMA and TACI. Does not bind to proteoglycans. Purity: >95% (SDS-PAGE). APRIL is a cytokine that belongs to the TNF superfamily and binds to TACI and BCMA. It is implicated in the regulation of tumor cell growth, is involved in monocyte/macrophage-mediated immunological processes and functions as an important survival factor for plasmablasts and bone marrow plasma cells. MultimericAPRIL™ is a high activity construct in which two trimeric APRIL ligands are artificially linked via the collagen domain of ACRP30. This construct very effectively stimulates proliferation B cell. A basic amino acid sequence (QKQKKQ) close to the N-terminus of APRIL is required for binding to negatively charged sulfated glycosaminoglycan side chains of proteoglycans. Proteoglycans mediate binding of APRIL to tumor cells as well as primary lymphoid cells.

 

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