ABTS 1-Component HRP Microwell Substrate (SUBA)

  ICT-6278 100 mL

Data Sheet:     SDS:    

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1X  

2-8°C  

3 years from date of manufacture  

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Description

 

ABTS 1-Component HRP Microwell Substrate is a ready-to-use solution containing 2,2’-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) in a mildly acidic buffer. This substrate is ideal for use in ELISAs where the detection level is in the µg-ng/mL range and horseradish peroxidase (HRP) is the conjugated detection enzyme. ABTS 1-Component HRP Microwell Substrate is suitable for ELISAs where the test samples contain high concentrations of the target molecule or when it is advantageous to utilize lower sample dilution factors. - ABTS 1-Component HRP Microwell Substrate is a ready-to-use solution containing 2,2’-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) in a mildly acidic buffer. This substrate is ideal for use in all ELISAs where the target detection level is in the µg-ng/mL range and horseradish peroxidase (HRP) is the conjugated detection enzyme. ABTS 1-Component HRP Microwell Substrate is suitable for ELISAs where the test samples contain high concentrations of the target molecule or when it is advantageous to utilize lower sample dilution factors., , ABTS substrate is oxidized by the peroxidase enzyme to yield a soluble blue-green reaction product. In endpoint assays, the reaction can be stopped by adding equal volumes of 1% sodium dodecyl sulfate (SDS)., , Bulk volumes are available upon request. - 1. Run ELISA according to the specific protocol through the conjugate incubation step., 2. Wash the wells three or four times with 1X ELISA Wash Buffer (catalog #652) to remove any residual, AP-conjugate., 3. Bring SUBP to room temperature; protect from light., 4. Pipette 100 µL SUBP into each well of the plate., 5. Incubate SUBP 10-60 minutes. Monitor the color intensity., 6. Read the plate between 405-420 nm and analyze. Alternatively, stop the reaction by adding 100 µL/well STOPP (catalog #6284) and read the samples between 405-420 nm within 1 hour.

 

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