2',7'-Dichlorofluorescin diacetate
CDX-D0064-G001 1 g
Synonyms: H2DCFDA; 2',7'-Dichlorodihydrofluorescein diacetate; Dichlorofluorescin DA; DCFDA; DCFH; DCFH-DA
Data Sheet: 
CAS
4091-99-0
Purity
>95% (HPLC)
MW
487.29
Shipment
Ambient
Short Term Storage
+4°C
Long Term Storage
-20°C
Shelf Life
Stable for at least 2 years after receipt when stored at -20°C.
Description
Chemical. CAS: 4091-99-0. Formula: C24H16Cl2O7. MW: 487.29. Dichlorofluorescin diacetate (H2DCFDA) is a chemically reduced analog of fluorescein, used as an indicator for reactive oxygen species (ROS) in cells. The cell permeable non-fluorescent H2DCFDA is cleaved by cellular esterases and oxidation, and converted to the highly fluorescent 2',7'-dichlorofluorescein. H2DCFDA might be reactive toward a broad range of oxidizing reactions that may be increased during intracellular oxidant stress. Peroxynitrite is an efficient mediator of this oxidation and neither NO, superoxide, nor hydrogen peroxide alone appear to oxidize DCFH. This probe is widely used to monitoring cellular redox processes. Used as a component/substrate of the 2′,7′-dichlorofluorescein diacetate (DCFH-DA) assay to quantitate reactive oxygen species (ROS). Can detect reactive oxygen species in live cells. Spectral data: lambdaEx=504nm/lambdaEm=529nm.
ESCLUSIVAMENTE PER USO DI RICERCA (RUO) e non per uso terapeutico o diagnostico su uomini o animali. Il prodotto NON è un Dispositivo Medico o un Diagnostico in Vitro.
PRODUCT FOR RESEARCH USE ONLY (RUO) and not for therapeutic or diagnostic use on humans or animals. The product is NOT a Medical Device or an In-Vitro Diagnostic (IVD).
