Vinci-Biochem Srl
Via Ponte di Bagnolo, 10
50059 Vinci (Firenze) Italy
vb@vincibiochem.it
Tel: +39 0571 568 147 -
 0571 568 135
Fax: +39 0571 568132 - P. IVA 05706610481

Elispot


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ELISpot and FluoroSpot – methods for SARS-CoV-2 vaccine development

 

3850-4HPW-R1-1
 
ELISpotPLUS for enumeration of cells IgG antibodies specific for SARS-CoV-2 RBD
This kit is ideal for users who want a convenient and sensitive assay. The assay is designed for enumeration of B cells secreting IgG antibodies specific for the SARS-CoV-2 Receptor Binding Domain (RBD). Enumeration of all cells secreting IgG (total IgG) can also be made.
The kit includes one ELISpot strip plate pre-coated with monoclonal anti-human IgG antibodies, recombinant RBD-WASP (recombinant RBD with site specific peptide tag WASP), biotinylated detection mAb, anti-WASP-HRP, Streptavidin-HRP, polyclonal activators, and ready-to-use TMB substrate. Pre-coated plates reduce assay time and minimize variability.
For memory B cells, we recommend pre-stimulation with R848 and recombinant human IL-2 (both included).
 
 
 

EliSpot Mabtech

MabTech Elispot

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Find 1 cell in 100,000 with ELISpot

ELISpot is a sensitive assay used to quantify cytokine- or immunoglobulin-secreting cells at the single-cell level.

• Sensitive detection of antigen-specific cytokine responses

• Investigation of vaccine efficacy and evaluation of novel vaccine adjuvants

• Characterization of cytotoxic T-cell activity by IFN-γ, granzyme B, and perforin analysis

• Quantification of antigen-specific memory B cells and vaccine-induced antibody responses

FluoroSpot Mabtech

Mabtech FluoroSpot

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Discover more with FluoroSpot

FluoroSpot combines the sensitivity of ELISpot with the capacity to analyze secretion of several analytes simultaneously.

       
     
 

 





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    Vinci-Biochem Srl
    Via Ponte di Bagnolo, 10
    50059 Vinci (Firenze) Italy
    vb@vincibiochem.it
    Tel:+39 0571 568147
    Fax:+39 0571 568132
    P. IVA 05706610481
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