
Reactivity
Budding Yeast, Human, Wide Range Predicted
Applications
ChIP, ChIP-Seq, CUT&RUN, DB, ICC, IF, WB
Source / Host
Rabbit
Immunogen
This Histone H3 trimethyl Lys4 antibody was raised against a peptide including trimethyl-lysine 4 of histone H3.
Shipment
Room Temp
Short Term Storage
-20C
Long Term Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years.
Description
Applications Validated by Active Motif: ChIP: 3 ug per ChIP ChIP-Seq: 4 ug each ICC/IF: 1 - 2 ug/ml dilution WB*: 2 - 4 ug/ml dilution CUT&Tag: 1 ul per 50 ul reaction* CUT&RUN: 1 ul per 50 ul reaction *This antibody has been validated for CUT&Tag using Active Motif's CUT&Tag-IT Assay Kit, Catalog No. 53160. *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western Blot.
ESCLUSIVAMENTE PER USO DI RICERCA (RUO) e non per uso terapeutico o diagnostico su uomini o animali. Il prodotto NON è un Dispositivo Medico o un Diagnostico in Vitro.
PRODUCT FOR RESEARCH USE ONLY (RUO) and not for therapeutic or diagnostic use on humans or animals. The product is NOT a Medical Device or an In-Vitro Diagnostic (IVD).