CFTR – HEK 293 Cell Line                                                                                                                                                  

BPS-60506

Cystic fibrosis transmembrane conductance regulator (CFTR) is a protein that in humans is encoded by the CFTR gene. CFTR is an ABC transporter-class ion channel that transports chloride and thiocyanate ions across epithelial cell membranes. Mutations of the CFTR gene affect functioning of the chloride ion channels in these cell membranes, leading to cystic fibrosis [1]. It is characterized by the triad of chronic bronchopulmonary disease (with recurrent respiratory infections), pancreatic insufficiency (which leads to malabsorption and growth retardation) and elevated sweat electrolytes [2].

Description: The CFTR-HEK293 Cell Line expresses full length, human cystic fibrosis transmembrane conductance regulator (CFTR) protein (Genbank # P13569). The expression of CFTR is confirmed by Western blotting.

Sequence: A synthetic codon-optimized DNA sequence encoding human CFTR protein [2] with C-terminal Streptavidin-Binding Peptide (SBP) [3] tag is stably integrated in 293HEK-Trex cells.

Application

• Drug compound screening

• Functional Assays

• Efficient antigen for mouse immunization

 

References:

1. Gadsby, D.C., et al. Nature 440 (7083): 477–483 (2006).

2. Hillier, L.W., et al. Nature 424:157-164 (2003).

3. McCann, C. M., et al. BioTechniques 38 (6): 945–952 (2005).

 

 

hTMEM16A (ANO1) - HEK293 Recombinant Cell line                                                                                                               

BPS-90230

Recombinant HEK293 cell line expressing human TMEM16A (transmembrane protein 16A, also called as anoctamin 1, calcium-activated chloride channel (ANO1), accession number NM_018043).

Each vial contains 1 X 10^6 cells in 1 ml of 10% DMSO.

Mycoplasma Testing: The cell line has been screened using the PCR-based VenorGeM Mycoplasma Detection kit (Sigma-Aldrich) to confirm the absence of Mycoplasma species.

Introduction: Calcium-activated chloride channels (CaCCs) are major regulators in numerous physiological processes including sensory transduction, epithelial secrection, cardiac and neutonal excitation, and smooth muscle contraction.

TMEM16A (ANO1), a member of a family of putative plasma membrane proteins, is identified as a CaCC that is activated by intracellular Ca2+ and Ca2+-mobilizing stimuli. It has eight putative transmembrane segments without domains evidently involved in calcium regulation. The relative permeability of TMEM16A to monovalent anions is NO3- > I- > Br- > Cl- > F-.

 

References:

Verkman A.S. and Galietta L.J.V. (2009) Cholide channels as drug targets. Nature Reviews 8: 153-171

Caputo A. et al. (2008) TMEM16A, a membrane protein associated with calciumdependent chloride channel activity. Science 322: 590-594

Yang Y.D. et al. (2008) TMEM16A confers receptor-activated calcium-dependent chloride conductance. Nature 455: 1210-1215

Schroeder B.C. et al. (2008) Expression cloning of TMEM16A as a calcium-activated chloride channel subunit. Cell 134: 1019-1029