Proteostasis is what every cell seeks: a balanced state in which all
proteins coexist and interact to optimal effect. When the proteostasis
network is in equilibrium, it is a hallmark of a healthy cell and a
healthy organism. When the network becomes unbalanced, through chemical
modification, physiological stress, aging, or disease, problems arise.
Browse Proteostasis Resources |
|
MMP RED and GREEN Drug Discovery Kits
|
|
-
Featuring Sensitive, Long-wavelength Fluorogenic Substrates
-
Improved red-shifted substrate with better kinetics and brighter
signal, allowing for lower substrate concentrations and resulting in
less compound interference
-
Includes active recombinant enzyme, substrate, and assay buffer
-
Convenient real-time kinetics of cleavage is easily determined
-
Microplate format for high-throughput screening
Scarica PDF
|
The MMP RED and GREEN Drug Discovery Kits are complete assay systems
designed to screen MMP inhibitors, using the quenched fluorogenic
substrate OmniMMP RED, or, in the case of MMP-3, MMP-3 Fluorogenic
Substrate. The assays are performed in a convenient 96-well microplate
format, with the compound NNGH also included as a prototypic control
inhibitor.
The new OmniMMP RED and MMP-3 Fluorogenic Substrates offer key
advantages over other MMP substrates.
1.Emission at the higher end of the spectrum (576nm and 521nm,
respectively, after excitation at 545nm and 494nm) avoids the
interference at lower wavelengths often exhibited by screening
compounds, and by substances commonly found in biological samples and
tissue culture medium.
2.Highly quenched (very low background), but once cleaved by MMPs emits
extremely bright signal.
3.MMP substrate peptides inherently display poor aqueous solubility,
often with Kms near their limits of solubility, making enzyme and
inhibitor kinetics difficult. MMP Kms for OmniMMP RED and MMP-3
Fluorogenic Substrates are well below the solubility limits of the
substrates.
4.In addition to the efficient binding as exhibited by low Kms, OmniMMP
RED and MMP-3 Fluorogenic Substrates are avidly cleaved by MMPs, with
kcat/Kms in the range of 105-107 M-1sec-1.
5.Better kinetics allows lower substrate concentrations, avoiding
inhibition by the substrate or competition with the inhibitor at the
active site.
6.The ultra-strong fluorescence of OmniMMP RED and MMP-3 Fluorogenic
Substrates allows for substrate concentrations much lower than the Km, a
condition generally desirable in inhibitor screening assays.
|
|
Ubiquitin and Ubiqutin-like Proteins (Ubls)
UbiQapture-Q Kit
An efficient tool to
Isolate and detect a range of specific ubiquitinylated protein
conjugates from cell extracts or tissue lysates and to purify ubiquitinylated proteins from cell-free in vitro assays
The UbiQapture-Q matrix has superior binding characteristics compared
to other commercially available matrices. In contrast to other kits that
permit only the capture of long polyubiquitin chain-conjugated proteins,
this product allows complete isolation of a wide range of
ubiquitin-protein conjugates from a specific lysate.
Highly stable UbiQapture-Q affinity matrix.
Minimal non-specific binding.
Facilitates isolation of both mono- and poly-ubiquitinylated proteins.
Release free proteins in their active/native form by cleavage from the
UbiQapture-Q matrix.
Compatible with a wide range of lysis buffers and cell/tissue samples.
New Products:
|
