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BioArray HighYield RNA Transcript Labeling Kit
The BioArray HighYield RNA Transcript Labeling Kit is commonly used in
conjunction with Affymetrix GeneChip arrays and other gene expression analysis
platforms. This kit provides reagents for the T7 RNA polymerase-based
transcription, biotin-labeling and amplification of cRNA for use in gene array
assays. Each reaction has been formulated for approximately 1 g of cDNA template
or a control template such as plasmid DNA (0.4 pmol).
Catalog Number: 42655-10 10 reactions
/
Content:
10x Biotin-labeled Ribonucleotides, 10X concentrated mixture of ATP, CTP, GTP,
UTP, Bio-UTP and Bio-CTP
10x RNase Inhibitor Mix, 20 units/l in Storage Buffer
20x T7 RNA Polymerase, 50 units/l in Storage Buffer
10x DTT, 100mM DTT
10x T7 HY Reaction Buffer, Tris buffered NaCl, MgCl2 and spermidine, with
stabilizer
BioArray Eukaryotic Hybridization Controls
Biotinylated control RNA sequences for assaying quality and performance of
Affymetrix GeneChip Eukaryotic Expression Assays. Includes bioB, bioC, bioD, cre
and Control Deoxyoligo B2.
Catalog Number: 42661-150 150 reactions
/ 42661-30 30 reactions
BioArray Deoxyoligo B2
The Enzo BioArrayTM Eukaryotic Control Deoxyoligo B2 is a synthetic biotin-labeled
sequence utilized in eukaryotic expression arrays by aiding software programs
such as the Affymetrix Microarray Suite in aligning the grid with the array
image. The Control Deoxyoligo B2 is supplied as a 3 nM solution.
Catalog Number: 42662-30 30 reactions
/ 42662-150 150 reactions
BioArray Single-Round RNA Amplification and Biotin Labeling System
The Enzo BioArray Single-Round RNA Amplification and Biotin Labeling System
provides an optimized protocol and reagents for the production of amplified
biotin-labeled aRNA from total cellular RNA samples.
The BioArray Single-Round RNA Amplification and Biotin Labeling System
includes kits for (1)cDNA synthesis (cat #42406), (2) cDNA purification (cat
#42407), and (3) in vitro transcription labeling (BioArray High Yield, cat
#42655).
Catalog Number: 42420-10 10 reactions
BioArray cDNA Synthesis Kit
The Enzo BioArray cDNA Synthesis Kit provides an optimized protocol and reagents
for the synthesis and purification of double-stranded cDNA from total cellular
RNA samples. The polyA+ mRNA in the total RNA is primed with a T7-oligo-dT
primer to synthesize the first strand of cDNA which proceeds by the action of
reverse transcriptase. Second
strand synthesis is accomplished by the combined activity of RNase H and DNA
polymerase. The cDNA purification
kit, included with the cDNA synthesis kit, removes nucleotides, primers, enzymes
and salts. The resulting purified
T7-promoter containing double-stranded cDNA can be used as template for in vitro
transcription with T7 RNA polymerase.
Catalog Number: 42406-10 10
reactions
Content:
cDNA Elution Tubes, 10
cDNA Purification columns, 10
Collection Tubes, 10
Control RNA (100 ug/mL), 10 uL
DNA Polymerase, 50 uL
dNTP Mix, 40 uL
DTT, 40 uL
First Strand Buffer, 40 uL
Nuclease-free Water, 1.5 mL
Promoter Primer, 10 uL
Reverse Transcriptase, 10 uL
RNase H, 10 uL
RNase Inhibitor, 10 uL
Second Strand Buffer, 150 uL
5X
cDNA Binding Buffer, 4.5 mL
BioArray Single-Round RNA Amplification and Biotin Labeling Kit without cDNA
Purification Reagents, Ver.2
The Enzo BioArray Single-Round RNA Amplification and Biotin Labeling System
provides an optimized protocol and reagents for the production of amplified
biotin-labeled aRNA from total cellular RNA samples.
The BioArray Single-Round RNA Amplification and Biotin Labeling System
includes kits for (1)cDNA synthesis (cat #42406) and (2) in vitro transcription
labeling (BioArray High Yield, cat #42655).
Catalog Number: 42421-10 10
reactions
CGH Labeling Kit for Oligo Arrays
Enzo's CGH Labeling Kit for Oligo Arrays has been optimized to label genomic DNA
for dual sample comparative genomic hybridization assays on Agilent
oligonucleotide arrays. All
required reagents are provided including fully optimized nucleotide mixes that
contain either cyanine 3-dUTP or cyanine 5-dUTP.
Catalog Number: 42671
CGH Labeling Kit for BAC Arrays
The CGH Labeling Kit for BAC Arrays has been optimized to label DNA for dual
sample comparative genomic hybridization (CGH) assays on BAC arrays.
This kit is specifically tailored to provide the flexibility required by
different laboratories and supplies sufficient reagents for two different
protocols. For the average user,
the Standard Protocol calls for a 4 hour labeling in a 50 ul reaction volume.
For users with dilute DNA (less than 20 ng/uL) or who prefer an overnight
labeling step, the Dilute DNA Protocol calls for a 16-hr labeling in a total
volume of 100 ul. The CGH Labeling Kit for BAC Arrays is a robust and consistent
system that enables detection of chromosomal alterations throughout the genome
with high sensitivity.
Catalog Number: 42670
Cyanine 3-dUTP Nucleotide Mix,
Klenow DNA Polymerase, 20 l
Primers/Reaction Buffer,
Cyanine 5-dUTP Nucleotide Mix,
Nuclease-free Water, 1 mL
Stop Buffer, 100 L
Genomic DNA Amplification: BioScore Screening and Amplification Kit
The BioScoreTM Screening and Amplification kit is a rapid and reliable method to
determine the quality of genomic DNA isolated from formalin-fixed
paraffin-embedded (FFPE) tissue samples before use in array-based genome-wide
copy number analysis and other genomic profiling applications. This dual purpose
kit utilizes a novel whole genome amplification (WGA) method to identify genomic
DNA samples that are suitable for microarray analysis prior to labeling and to
predict sample performance with virtually 100% concordance.
After prescreening, the amplified genomic DNA can then be used in genomic
profiling assays such as array analysis.
Catalog Number:
BioArray Terminal Labeling Kit with Biotin-ddUTP for DNA Probe Array Assays
The BioArray Terminal Labeling Kit is commonly used for DNA analysis
microarrays. This kit provides reagents for biotin labeling of the 3' termini of
cDNA for use in gene array assays. Each reaction will label up to 100 picomoles
of amplified and fragmented target cDNA.
Catalog Number:
100x Biotin-ddUTP, 25 l
10x CoCl2 Solution, 250 l
50x Terminal Transferase, 50 l : Terminal Deoxynucleotide Transferase in storage
Buffer
5x Reaction Buffer, 500 l : Potassium cacodylate buffer, pH 7, containing -mercaptoethanol
and stabilizer
Allylamine-dUTP
Allylamine-labeled DNA can be efficiently conjugated to the Cyanine 3 or 5-NHS
Ester for indirect labeling in microarray analysis.
The efficient incorporation of Allylamine-dUTP, in contrast to many dye-labeled
nucleotides, provides for greater incorporation of cyanine esters and stronger
signals.
Catalog Number: 42861 2.5 umol, 50
ul
Cyanine 5-NHS Ester Pack
Enzo's Cyanine 5-NHS Ester Pack can be used for indirect or post labeling of
nucleic acids in spotted microarray analysis.
Highly efficient nucleic acid labeling can be achieved by using an NHS
Ester of a cyanine dye to label nucleic acids that have been modified by the
incorporation of reactive allylamine groups.
Catalog Number:
Cyanine 3-NHS Ester Pack
Enzo's Cyanine 3-NHS Ester Pack can be used for indirect or post labeling of
nucleic acids in spotted microarray analysis.
Highly efficient nucleic acid labeling can be achieved by using an NHS
Ester of a cyanine dye to label nucleic acids that have been modified by the
incorporation of reactive allylamine groups.
Catalog Number:
Cyanine-3-dUTP, 25 nmol
Cyanine-3-dUTP, 1mM, is a highly fluorescent nucleotide analog that can be
incorporated by a variety of DNA polymerases. It is ideal for labeling DNA
probes, oligodeoxynucleotides and other nucleic acids where it replaces TTP in
reactions catalyzed by E. coli DNA polymerase I (holoenzyme and Klenow
fragment), T4 DNA polymerase, Taq DNA polymerase, and Mu-MLV and AMV reverse
transcriptases. It can also be incorporated in 3'-terminal labeling reactions
using terminal deoxynucleotide transferase. Cyanine labeled DNA can be produced
by reverse transcription, nick translation, random primed labeling and
3'-terminal labeling reactions. The GeneBeam Cyanine-3-dUTP, which fluoresces in
the orange region of the spectrum, is a useful label for multicolor analyses. It
can be used in conjunction with GeneBeam Cyanine-5-dUTP for first strand cDNA
labeling in dual sample gene array assays.
Catalog Number:
Cyanine 3-NHS Ester Pack
Enzo's Cyanine 3-NHS Ester Pack can be used for indirect or post labeling of
nucleic acids in spotted microarray analysis.
Highly efficient nucleic acid labeling can be achieved by using an NHS
Ester of a cyanine dye to label nucleic acids that have been modified by the
incorporation of reactive allylamine groups.
Catalog Number:
Cyanine-5-UTP
Cyanine-5-UTP is a highly fluorescent nucleotide analog that can be incorporated
into RNA by a variety of RNA polymerases, including T7 RNA polymerase.
The dye-labeled transcripts produced by these reactions are used as
probes in a wide variety of applications, including microarray assays.
Catalog Number:
Cyanine-3-UTP
Cyanine-3-UTP is a highly fluorescent nucleotide analog that can be incorporated
into RNA by a variety of RNA polymerases, including T7 RNA polymerase.
The dye-labeled transcripts produced by these reactions are used as a
probe in a wide range of applications, including microarray assays.
Catalog Number:
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