Lipid Peroxidation

8- iso Prostaglandin F2a  EIA Kit (Cayman) - Code 850-205

[8-Isoprostane EIA Kit (Cayman)]

Lipid Hydroperoxide (LPO) Assay Kit (Cayman) (100 determinations) - Code 850-026

Any sample containing lipid hydroperoxide can be easily and reliably quantified spectrophotometrically using either glass or quarz 1ml cuvettes. Sensitivity: 0.25-5nmol hydroperoxide per assay tube.

Lipid Hydroperoxide (LPO) Assay Kit (Cayman) (96 wells) - Code 850-203

Same Kit as Prod. No. 850-026-KI01 but contains additionally a reusable glass 96 well plate (for use of organic solvents). 

850-287-KI01 1 Kit

Quantity: 160 tests.

The sensitivity of measuring Thiobarbituric Acid Reactive Substances (TBARS) has made this assay the method of choice for screening and monitoring lipid peroxidation, a major indicator of oxidative stress. This rapid, easy-to-use procedure has been modified by researchers for use with many types of samples including drugs, food products and human and animal biological tissues. The assay has provided important information regarding free radical activity in disease states and has been used for measurement of antioxidant activity of several compounds. The kit is designed to provide stanardized, reproducible results. Principles of the Kit: Malondialdehyde (MDA) forms a 1:2 adduct with thiobarbituric acid. The adduct can be measured by fluorometry or spectophotometry. Biological specimens contain a mixture of thiobarbituric acid reactive substances (TBARS), including lipid hydroperoxides and aldehydes, which increase as a result of oxidative stress. TBARS return to normal levels over time, depending upon the presence of anti-oxidants. In this assay, an MDA standard is used to construct a standard curve against which unknown samples can be plotted.

For further information please request the Product Information Sheet/Manual.

(E)-4-Hydroxynonenal - Code 270-245

Among the aldehydes formed, HNE is the major product of lipid peroxidation, and it has been suggested to play a major role in liver toxicity associated with lipid peroxidation. Prevents NF-kB activation and TNF expression by inhibiting IkB phosphorylation and subsequent proteolysis.

(E)-4-Hydroxynonenal-dimethylacetal (Nuova forma Stabile del 4-Hydroxynonenal)

ALX-270-375 

1 vial (Liquid. 6.8mg in 1ml n-hexane. Yields ~5.2mg aldehyde after hydrolysis)

Antiserum to (E)-4-Hydroxy-nonenal [HNE] - Code 210-767

From rabbit. Antigen: Free HNE coupled to KLH. Specificity: To visualize HNE-adducts. Application: WB, ELISA.

Carazostatin, Streptomyces chromofuscus - Code 350-253

Potent inhibitor of lipid peroxidation induced by free radicals in rat brain homogenates (IC 50 =170nM) and lipid peroxidation in mouse plasma (ID50 =21.7mg/kg; oral).

Pyrrolostatin, Streptomyces chrestomyceticus - Code 350-252

Potent inhibitor of lipid peroxidation. IC 50 =49M (inhibition of lipid peroxidation in rat brain homogenate).