Interferon

Monoclonal Antibodies

Clone

Size

Product No.

Mouse Anti Hu-IFN-a, neutralizing

MMHA-2

50ug

21100-1

Mouse Anti Hu-IFN-a2, neutralizing

MMHA2

500ug

21100-2

Mouse Anti Hu-IFN-a2, NON-neutralizing

MMHA-3

50ug

21101-1

Mouse Anti Hu-IFN-a2, neutralizing

MMHA-1

50ug

21105-1

Mouse Anti Hu-IFN-a, neutralizing

MMHA-8

50ug

21110-1

Mouse Anti Hu-IFN-a, neutralizing

MMHA-11

50ug

21112-1

Mouse Anti Hu-IFN-a, neutralizing

MMHA-13

50ug

21116-1

Mouse Anti Hu-IFN-aD, neutralizing

MMHA-6

50ug

21125-1

Mouse Anti Hu-IFN-aD, neutralizing

MMHA-9

50ug

21127-1

Mouse Anti Hu-IFN-aD, neutralizing

MMHA-14

50ug

21129-1

Mouse Anti Hu-IFN-a, NON-neutralizing, cell supernatant

MMHA-10

50ug

21154-1

Mouse Anti Hu-IFN-a/bReceptor Chain2 (beta chain) [CD118]

MMHAR-2

50ug

21385-1

Mouse Anti Hu-IFN-w, neutralizing

OMG-4

100ug

21395-1

Mouse Anti Hu-IFN-b, neutralizing

MMHB-3

50ug

21400-1

Mouse Anti Hu-IFN-b, neutralizing

MMHB-1

50ug

21405-1

Mouse Anti Hu-IFN-b, neutralizing; cell supernatant

MMHB-11

50ug

21445-1

Mouse Anti Hu-IFN-b, neutralizing; cell supernatant

MMHB-12

50ug

21450-1

Mouse Anti Hu-IFN-b, neutralizing; cell supernatant

MMHB-13

50ug

21455-1

Mouse Anti Hu-IFN-b, neutralizing; cell supernatant

MMHB-14

50ug

21460-1

Mouse Anti Hu-IFN-b, neutralizing; cell supernatant

MMHB-15

50ug

21465-1

Mouse Anti Hu-IFN-b, neutralizing; cell supernatant

MMHB-16

50ug

21470-1

Mouse Anti Hu-IFN-g, neutralizing

MMHG-1

500ug

21500-1

MAb to IFN-g (human)  Purified

45.15

100ug

MAb to IFN-g (human)  Biotin

45.15

100ug

MAb to IFN-g (human)  FITC

45.15

120 tests

MAb to IFN-g (human)  R-PE

45.15

120 tests

Polyclonal Antibodies

Sheep Anti Human IFN-a

1x10⁵nu

31100-1

Rabbit Anti Human IFN-a

1x10⁵nu

31101-1

Rabbit Anti Human IFN-a

2x10⁴nu

31130-1

Rabbit Anti Human IFN-a/b Receptor Chain 2 (beta chain)

50ug

31385-1

Sheep Anti Human IFN-b

2x10⁴nu

31400-1

Rabbit Anti Human IFN-b

2x10⁴nu

31410-1

Rabbit Anti Human IFN-g

2x10⁴nu

31500-1

Rabbit Anti Hu-IFN-g Receptor Chain 1 (alpha chain) [CD 119]

200ug

31580-1

Rabbit Anti Hu-IFN-g Receptor Chain 2 (beta chain) [CD 119]

50 ug

31585-1

Rabbit Anti Human IL-10 Receptor Chain 2 (beta chain)

50ug

31852-1

Rabbit Anti Mouse IFN-a

2x10⁴nu

32100-1

Sheep Anti-Mouse IFN-a/b

2x10⁴nu

32105-1

1x10⁵nu

32105-2

Rabbit Anti Mouse IFN-b

2x10⁴nu

32400-1

Rabbit Anti Mouse IFN-g

2x10⁴nu

32500-1

Rabbit Anti Mouse IFN-g Receptor Chain 1 (alpha chain) [CD119]

50ug

32580-1

Rabbit Anti Mouse IFN-g Receptor Chain 2 (beta chain) [CD119]

50ug

32585-1

Rabbit Anti Rat IFN-b

500ug

33400-1

Rabbit Anti Rat IFN-g

200ug

33500-1

Rabbit Anti Pig IFN-a

2x10⁴nu

37100-1

Rabbit Anti Pig IFN-g

2x10⁴nu

37500-1

Sheep Anti Human IFN-a

1x10⁵nu

31100-1

Rabbit Anti Human IFN-a

1x10⁵nu

31101-1

Rabbit Anti Human IFN-a

2x10⁴nu

31130-1

Rabbit Anti Human IFN-a/b Receptor Chain 2 (beta chain)

50ug

31385-1

Sheep Anti Human IFN-b

2x10⁴nu

31400-1

Rabbit Anti Human IFN-b

2x10⁴nu

31410-1

Rabbit Anti Human IFN-g

2x10⁴nu

31500-1

Rabbit Anti Hu-IFN-g Receptor Chain 1 (alpha chain) [CD 119]

200ug

31580-1

Rabbit Anti Hu-IFN-g Receptor Chain 2 (beta chain) [CD 119]

50 ug

31585-1

Rabbit Anti Human IL-10 Receptor Chain 2 (beta chain)

50ug

31852-1

Rabbit Anti Mouse IFN-a

2x10⁴nu

32100-1

Sheep Anti-Mouse IFN-a/b

2x10⁴nu

32105-1

1x10⁵nu

32105-2

Rabbit Anti Mouse IFN-b

2x10⁴nu

32400-1

Rabbit Anti Mouse IFN-g

2x10⁴nu

32500-1

Rabbit Anti Mouse IFN-g Receptor Chain 1 (alpha chain) [CD119]

50ug

32580-1

Rabbit Anti Mouse IFN-g Receptor Chain 2 (beta chain) [CD119]

50ug

32585-1

Rabbit Anti Rat IFN-b

500ug

33400-1

Rabbit Anti Rat IFN-g

200ug

33500-1

Rabbit Anti Pig IFN-a

2x10⁴nu

37100-1

Rabbit Anti Pig IFN-g

2x10⁴nu

37500-1

Polyclonals Bead Based Quantification (BBQ) Assay Kits

BBQ Assays - Principle

The BBQ Assay is a particle based immunoassay for the quantitative detection of cytokines and other cell communication factors (like those involved in apoptosis). Antibody-coated particles in the close environment of cells bind to the antigen before any dilution into the supernatant occurs. The protein bound to the particles is now stained with a second, fluorescence-labelled antibody forming a sandwich layer and the particles are analysed by flow cytometry. The fluorescence shift of the particles is proportional to the amount of protein trapped by the particles. The ease of use of the magnetic particles enables the detection of protein in the presence of cells.

IFN-g (human) BBQ Assay Kit

HZ-H100914-KI01

IFN-g (mouse) BBQ Assay Kit

HZ-M100914-KI01

Advantages:

No enzyme reaction involved:

Intra- and interassay variation is very small. Considerably less washing steps are required than in ELISA. Wider detection range makes

dilution of the samples unnecessary.

Extremely flexible:

Sample volume, cell number and incubation time can be varied to achieve the desired detection range.

Detects signals from various matrices:

Cell culture media, sera and plasma can be used.

Conservation of the signal:

Since the antibodies are covalently bound to the particles (not adsorbed as on an ELISA plate), the incubation and storage time of the

antigen loaded particles is only limited by the stability of the antigen.

Quick and easy handling:

Magnetic particles make washing very easy, no relevant loss of particles occurs.

Multiparameter analysis possible:

Up to 3 analytes per sample can be measured in one run.

General Literature References:

Cytokine immunotrapping: an assay to study the kinetics of production and consumption or degradation of human interferon-gamma: A.C. Akdis, et al.; J. Immunol. Methods 182, 251 (1995)/ Detection and differentiation of platelet-specific antibodies by flow cytometry: the bead-mediated platelet assay: W. Helmberg, et al.; Transfusion 37, 502 (1997)/ Magnetic bead enzyme-linked immunosorbent assay (ELISA) detects antigenspecific binding by phage-displayed scFv antibodies that are not detected with conventional ELISA: M. Kala, et al.; Anal. Biochem. 254, 263 (1997)/ The sensitive detection and quantitation of antibody to HCV by using a microsphere-based immunoassay and flow cytometry: T.M. McHugh, et al.; Cytometry 29, 106 (1997)