Gent. Cliente,

Vinci-Biochem e' lieta di presentarle FluoForte Calcium Assay Kit.  

 
Utilizzando il piu' brillante e sensibile indicatore per il Ca2+, il FluoForte™ Calcium Assay kit di ENZO, fornisce fornisce una intensita' di segnale molto piu' alta in high-throughput e la piu' ampia assay window.

	Intensita' di fluorescenza significativamente piu' brillante Ampiamente applicabile sia a target di GPCR che Calcium ion channel La piu' ampia assay window sul mercato Nessun quencher dye e' necessario Utilizzabile in High Throughput Screening Facile da usare, no-wash calcium mobilization assay
	Figure 1: Hela cells were seeded overnight at 40,000 cells per 100 µL per well in a 96-well black wall/clear bottom costar plate. The growth medium was removed, and the cells were incubated with 100 µl of 4 uM Fluo-3 AM, Fluo-4 AM or FluoForte™ AM in HHBS at 37 °C, 5% CO2 incubator for 1 hour. The cells were washed twice with 200 µl HHBS, ATP (20 µL/well) was added to achieve concentrations of 100 nM with dye efflux inhibitor, then immediately imaged with a fluorescence microscope (Zeiss) using FITC channel
	Fluoforte - HTS GPCRs Application   GPCR activation can be detected by direct measurement of the receptor mediated cAMP accumulation or changes in intracellular Ca2+ concentration. GPCR targets that couple via Gq produce an increase in intracellular Ca2+ that can then be measured with FluoForte and a fluorescence microplate reader. FluoForte™ has been validated on such instruments as FLIPRTETRA, Flex-Station® II and 3, Perkin Elmer Cellux®, and Hamamatsu FDSS6000/7000 models. Besides its robust applications for GPCR targets, our FluoForte™ Calcium Assay Kit can also be used for characterizing calcium ion channels and screening calcium ion channel-targeted compounds. FluoForte™ Calcium Assay Kit: FIGURE 1: FluoForte™ enters cell as a membrane permeable acetoxymethyl (AM) ester. Once inside the cell, FluoForte is hydrolyzed by intracellular esterases. Cell membrane impermeable, negatively charged form of FluoForte- is now capable of binding with Ca2+. Some cell lines express an organic anion transporter, which leads to the export of negatively charged FluoForte- . This can be prevented by adding Dye Efflux Inhibitor, a transport inhibitor   Fluoforte - Cellular Analysis Application     FIGURE 1: ATP Dose Response Curves in CHO-M1 cells. CHO cells were seeded overnight at 40,000 cells per 100 µl per well in a 96-well black wall/clear bottom microplate. (A) The cells were incubated with 100 µl of reagent from Life Technologies’ Fluo-4 Direct kit™, Molecular Devices’ Calcium 4 kit (both based upon manufacturer’s protocol) or Enzo’s FluoForte™ dye (B) The cells were incubated with reagent from Enzo’s FluoForte™ Calcium Assay kit, or with Life Technologies’ Fluo-4 NW kit (based upon manufacturer’s protocol). ATP (20 µl/well) was added by FlexStation to achieve the final indicated concentrations. No significant difference in EC50 of ATP for FluoForte™ assay kit, Fluo-4 Direct™ and Calcium 4 was observed (shown in A), and also comparable EC50 values were observed between FluoForte™ Assay kit and Fluo-4 NW (shown in B). In all cases FluoForte™ Assay kit generated the highest intensity signal.   FIGURE 2: Comparisons of FluoForte™ Assay Kit, Fluo-4 Direct™, and Calcium 4 detection of intracellular calcium mobilization in CHO-M1 cells. CHO cells were seeded overnight in 40,000 cells per 100 µl per well in a 96-well black wall/clear bottom costar plate. The cells were incubated with 100 µl of reagent from Life Technologies’ Fluo-4 Direct™ kit, Molecular Devices’ Calcium 4 kit (both based on manufactures’ protocol) or Enzo’s FluoForte™ Assay kit. ATP (20 µL/well) was added by FlexStation to achieve concentrations of 200 nM.
	Scopra questo prodotto
	FluoForte Calcium Assay Kit (HT) ENZ-51016  FluoForte Calcium Assay Kit (starter pack) ENZ-51017  FluoForte Reagent (5 x 50 g) ENZ-52014  FluoForte Reagent (1 mg) ENZ-52015   GFP-Certified™ FluoForte™ Reagent ENZ-52016 GFP-Certified™ FluoForte™ Calcium Assay Kit for microplates ENZ-51020   Scarica pdf
	Load dye in live cells at 37°C or room temperature: Room temperature is more compatible with HTS applications Homogeneous mix-and-read, no-wash calcium mobilization assay: Washing cells introduces errors Significantly brighter fluorescence intensity: 2X brighter than Fluo-4, Fluo-4 Direct, Fluo-4 NW, FLIPR® Calcium 4 assay kits. 4X brighter than Fluo-3 EC50 values (half maximal effective concentration): Comparable with Fluo-3, Fluo-4, Fluo-4 Direct™ and FLIPR® Calcium assay kits No quencher dye: No potential interference from compound ligand-receptor interactions Larger assay window than other dyes: Easily detects low calcium mobilization in challenging cell lines and receptors Maximum excitation @ ~490 nm: Readily adopted into established protocols and instrument workflows Stringently manufactured: Controls and eliminates non-specific assay artifacts
	Per ulteriori informazioni:   http://www.enzolifesciences.com   http://www.enzolifesciences.com/global-subpages/welcome/adwords/fluoroforte/fluoforte-calcium-assay-is-visibly-brighter    Disponibili da ENZO Life Sciences anche:    Calcein (Ultra Pure) ENZ-52001 100 mg Calcein AM (Ultra Pure) ENZ-52002 1 mg Calcein Blue (Ultra Pure) ENZ-52003 250 mg Fluo-3 AM (Ultra Pure) ENZ-52004 1 mg Fluo-3 (Ultra Pure) ENZ-52005 1 mg FURA-2 AM (Ultra Pure) ENZ-52006 1 mg FURA-2 (Ultra Pure) ENZ-52007 1 mg INDO-1 AM (Ultra Pure) ENZ-52008 1 mg INDO-1 (Ultra Pure) ENZ-52009 1 mg Rhod-2 AM (Ultra Pure) ENZ-52010 2 mg Rhod-2 (Ultra Pure) ENZ-52011 3 mg Quin-2 AM (Ultra Pure) ENZ-52012 4 mg Quin-2 (Ultra Pure) ENZ-52013 5 mg
	Tel 0571 568 147 - Fax. 0571 568 132 - vb@vincibiochem.it

* Enzo, Alexis, Biomol and FluoForte are trademarks of Enzo Life Sciences, Inc. FLIPR is a trademark of Molecular Devices. Fluo 4 Direct is a trademark of Invitrogen Corp. Several of Enzos products and product applications are covered by US and foreign patents and patents pending.