LavaCell

rapidly stain cellular membranes in live or fixed cells

LavaCell is a small, neutral, non-toxic compound that readily diffuses into live or fixed cells and spontaneously reacts to produce bright orange fluorescence. This naturally fluorescent compound is excited by a variety of common lasers and can be multiplexed with a wide range of common blue, green and yellow-emitting probes.

Cells require no pre-treatment or permeabilization for staining as LavaCell is a neutral molecule that crosses cell membranes. LavaCell rapidly reacts with free amine groups on proteins, which produces a bright orange fluorescent signal (Figure 1). LavaCell does not stain nucleic acids nor does it adversely affect cell growth. LavaCell can be excited with common laser sources (405, 488 and 532 nm) and has a Stokes shift of over 100 nm with peak fluorescence emission at 610 nm.

Advantages

• Water soluble fluorescent cell stain

• Fluoresces on interaction with proteins; no need to wash away unbound stain

• Readily permeates into cells

• No effect on cell growth or viability

• Ideal for multiplexing with other fluorophores due to long Stokes shift

Contents and Storage

200 g (1 mg/ml) in DMSO. Store at -20C in the dark. Contents guaranteed 6 months when stored properly.